Fig. 4 Exposure to two chromatic wavelengths recapitulates white light behavior. (A) Behavior rig set up for two LED experiment. (B) Visualization of combined wavelengths. (C) Representative local search tracks for dual cold white light controls (W/W), combined 415/530 nm, combined 415/660 nm, and combined 530/660 nm light exposure. Scale bar 10 mm. (D–L) Quantification for the light ON (D, G, J) and OFF (E, H, K) responses and fold change between photoperiods (F, I, L) for each motor metric. Asterisks indicate p ≤ 0.05 from white light control. N’s: W/W = 215, 415/530 nm = 94, 415/660 nm = 80, 530/660 nm = 63.
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