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Fig. 6 Acrylamide induces cardiomyocyte apoptosis via NOTCH-PI3K/AKT signaling in H9c2 cells. (A to D) Western blotting of apoptotic pathway related proteins (BCL-2, BAX, pro-Caspase 9, and cleaved Caspase 9) shows that acrylamide treatment activates apoptotic pathway in H9c2 cells (n = 3 per group). (E) Relative mRNA expression of NOTCH signaling pathway-related genes in H9c2 cells with acrylamide treatment for 48 h (n = 4 per group). (F and G) Western blotting of NOTCH1 and DLL1 proteins shows that acrylamide treatment inhibits NOTCH signaling pathway in heart of 5-month-old mice (n = 3 per group). (H) Schematic diagram of NOTCH signaling changes with acrylamide exposure. (I and J) Western blotting of p-AKT and AKT proteins shows that acrylamide treatment inhibits PI3K/AKT signaling pathway in heart of mice (n = 3 per group). Data are presented as the means ± SEM. Significance was calculated using 2-tailed P values by unpaired Student’s t test or one-way ANOVA with Tukey’s post hoc test; *P < 0.05, **P < 0.01, and ***P < 0.001; groups labeled with different letters differed significantly (P < 0.05). GAPDH, glyceraldehyde-3-phosphate dehydrogenase.