ZFIN Image: Ligunas et al., 2024, Fig. 2

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Figure Caption

Fig. 2 Split fluorescent protein labeling can be spatially restricted by transgenic expression of mNG21-10. A?F. Transgenic embryos expressing mNG21-10 under control of the fezf2 (A?B), myl7 (C?D), or ubb (E?F) promoters and injected with mNG211-H2B mRNA. A? shows the boxed region in A with brightness rescaled to demonstrate fluorescence is localized to nuclei. G?L. Transgenic embryos expressing GFP under control of the fez1 (G?H), myl7 (I?J), or ubb (K?L) promoters. Images were acquired at 24 h post-fertilization. Fluorescence images are maximum projections of confocal z-stacks. Scale bars in B, F, H, and L, 200 ?m. Scale bars in D, J, 50 ?m.

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Reprinted from Developmental Biology, 514, Ligunas, G.D., Paniagua, G., LaBelle, J., Ramos-Martinez, A., Shen, K., Gerlt, E.H., Aguilar, K., Nguyen, N., Materna, S.C., Woo, S., Tissue-specific and endogenous protein labeling with split fluorescent proteins, 109-116, Copyright (2024) with permission from Elsevier. Full text @ Dev. Biol.