ZFIN Image: Ligunas et al., 2024, Fig. 1

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Figure Caption

Fig. 1 Split fluorescent protein fragments are functional in zebrafish embryos. A. Schematic illustrating our protein labeling strategy using a split fluorescent protein. Transgenic (Tg) mNG21-10 is expressed under the control of a tissue-specific promoter (tsp) while mNG211 is inserted into protein-coding genes by CRISPR/Cas-directed gene editing. Fluorescence (green) is only generated in tissues co-expressing mNG21-10 and the mNG211-tagged protein of interest. B?E. Embryos were injected with GFP1-10 and GFP11-H2B (split-GFP, B) or mNG21-10 and mNG211-H2B (split-mNG2, C?E) mRNAs then imaged at 6 h post-fertilization (hpf) on a confocal microscope (B, C) or at 24 hpf on a fluorescence stereomicroscope (D, E). Confocal images are displayed as maximum z-projections. Scale bars in B and C, 50 ?m. Scale bar in E, 200 ?m.

Acknowledgments

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Reprinted from Developmental Biology, 514, Ligunas, G.D., Paniagua, G., LaBelle, J., Ramos-Martinez, A., Shen, K., Gerlt, E.H., Aguilar, K., Nguyen, N., Materna, S.C., Woo, S., Tissue-specific and endogenous protein labeling with split fluorescent proteins, 109-116, Copyright (2024) with permission from Elsevier. Full text @ Dev. Biol.