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Fig. 7 Riboflavin alleviates lens opacity in autophagy-related cataract models. (A) Representative images show the distribution of nuclei and F-actin in the lens of 72 hpf WT, cx50-deficient zebrafish, and cx50-deficient zebrafish treated with DMSO or riboflavin (80 µM). Scale bar: 10 µm. (B) Quantification of the severity of lens defects and the number of nuclei in lenses of each group (n > 25 zebrafish for each group) (Mann-Whitney test). (C) Representative images show that riboflavin alleviates lens opacity in the H2O2-induced cataract zebrafish model (n > 6 for each group). (D) Quantification of relief efficiency (Mann-Whitney test). (E) Representative images show that riboflavin could relieve cataract phenotype in cold-induced cataract rat models. (F) Quantification of transmittance (n > 5 for each group). Mean ± SD, *p < 0.05. N.S. not significant (Mann-Whitney test).