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Fig. 3 ATG16L1 is mainly degraded by the ubiquitin-proteasome system. (A) HLE cells were treated with 10 µM MG132 or 30 µM CQ for 6 h, and Western blotting was performed. (B) Quantification of LC3 levels, n = 3, *p < 0.05 (Mann-Whitney test). (C) HLE cells were treated with 30 µg/ml CHX for the indicated time, and the ATG16L1 expression was detected by Western blotting, n = 3. (D) ATG16L1 expression relative to GAPDH was quantified. (E) CX50-overexpressing HLE cells or (G) CX50-knockdown HLE cells were treated with 30 µM CHX for different times and (F and H) quantification of the ATG16L1 expression levels normalized to the GAPDH, n = 3, mean ± SD.