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Fig. 4 Expression of Harbinger-derived genes in zebrafish embryos and adults. (A) Relative gene expression determined by qPCR compared to the 18S housekeeping gene during embryonic development (from 1hpf to 3dpf). The MBT is shown with an arrow. (B) Relative gene expression determined by qPCR compared to the 18S housekeeping gene in female (F) and male (M) adult tissues. (C) Whole-mount in situ hybridization of MSANTD2 in zebrafish embryos from 1.25hpf to 24hpf using a sense probe as a control. The head or future head region is indicated with ‘h’. fb, forebrain; mb, midbrain; hb, hindbrain. Scale bars: 200 μm.