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Fig. 2 Comparative functional characterization of Cluster 1 FOXP2-HARs using transgenic zebrafishes. Fluorescent microphotographs showing the EGFP expression pattern driven by the human or chimpanzee ortholog of each FOXP2-HARs. In addition, bright field (A?C) and fluorescent (D?F) images of wild-type zebrafish are shown. ANC1198-Hs at 24 (G), 48 (H), and 72 hpf (I); ANC1198-Pt at 24 (J), 48 (K), and 72 hpf (L); HACNS506-Hs at 24 (M), 48 (N), and 72 hpf (O); HACNS506-Pt at 24 (P), 48 (Q), and 72 hpf (R); 2xHAR502-Hs at 24 (S), 48 (T), and 72 hpf (U); 2xHAR502-Pt at 24 (V), 48 (W), and 72 hpf (X); HACNS750-Hs at 24 (Y), 48 (Z), and 72 hpf (AA); HACNS750-Pt at 24 (AB), 48 (AC), and 72 hpf (AD); ANC1197-Hs at 24 (AE), 48 (AF), and 72 hpf (AG); ANC1197-Pt at 24 (AH), 48 (AI), and 72 hpf (AJ); HAR170-Hs at 24 (AK), 48 (AL), and 72 hpf (AM); HAR170-Pt at 24 (AN), 48 (AO), and 72 hpf (AP); HACNS169-Hs at 24 (AQ), 48 (AR), and 72 hpf (AS); HACNS169-Pt at 24 (AT), 48 (AU), and 72 hpf (AV). Only one selected transgenic line for each sequence is showed. All the Cluster 1 transgenic lines are shown in figure S4, Supplementary Material online. Note that the yolk sac (yolk) of the fishes emits autofluorescence. fb, forebrain (includes telencephalon, diencephalon, and thalamus); mb, midbrain (includes tectum); hb, hindbrain; sc, spinal cord; dig, digestive tube. Scale bar: 500 µm.