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Fig. 2 Expression pattern of nkx1.2. (A) Expression of nkx1.2 mRNA in different tissues detected by real-time PCR. (B) Expression profile of nkx1.2 at different stages of embryonic development as measured by real-time PCR. Fold difference was calculated as 2−ΔΔCt with beta-actin (Danio rerio, i.e., zebrafish) as a reference gene. Vertical bars represent the mean ± SEM (n = 3).
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