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Fig. 6 AS is altered in Rbfox1Δ2xHAR.321/Δ2xHAR.321 and Rbfox1Δ2xHAR.505/Δ2xHAR.505 mutant mice. (A) Types of AS events and the number of statistically significant events (FDR < 0.05; ΔPSI > 0.1) found in 2xHAR.321 and 2xHAR.505 knockout mice. (B) Pie chart showing the breakdown of AS events. (C) Proportion of differentially spliced genes with evidence of Rbfox1 direct binding as identified by iCLIP experiments from whole mouse brain at P15 (43). (D and E) Sashimi plots of selected examples of (D) exon skipping (Stx3) and (E) RI (Tmem175) in the Rbfox1Δ2xHAR.321/Δ2xHAR.321 mutant versus wild-type mice. Sashimi plots show the density of reads for each alternative exon or intron depicted below for each gene transcript. Arcs in the plots represent splice junctions that connect exons, and the number on each arc is the junction depth (number of junction reads). Important protein domains from UniProt are shown below the gene transcripts. Validation experiments for Stx3 and Tmem175 are included in fig. S10.