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Fig. 3 2xHAR.321 and 2xHAR.505 drive expression to rbfox1 cells in zebrafish. Representative transgenic zebrafish expressing EGFP under the control of (A) 2xHAR.321 human sequence and (B) 2xHAR.505 chimpanzee sequence at 72 hpf. Yellow bars indicate the approximate location of the coronal cryosections. (C to J) Confocal microscopy images of cryosections showing EGFP and rbfox1 expression on the [(C) and (D)] optic tectum, [(E) and (F)] thalamus, [(G) and (H)] retina, and [(I) and (J)] medulla oblongata. (K and L) Quantification of double-positive cells of total EGFP-positive or rbfox1-positive cells for (K) 2xHAR.321 and (L) 2xHAR.505. Arrows indicate examples of double-expressing cells (yellow), rbfox1-expressing cells (pink), and EGFP-expressing cells (light blue).