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Fig. 6 ZFU6-LentiCRISPRV2-EMX1 mediated gene editing in 293 T cell. A Genomic DNA structure and sgRNA site of EMX1. B PCR products of EMX1. M, DL2000 DNA marker; 1, cells transfected with ZFU6-LentiCRISPRV2; 2, cells transfected with ZFU6-LentiCRISPRV2-EMX1. C Sequencing of the EMX1 gene from cells with ZFU6-LentiCRISPRV2-EMX1 transfection and puromycin selection for 7 days. D The mutation type of EMX1 gene. Dash represents the deleted base, red letters indicate the CRISPR target site, green letters indicate the PAM sites and blue letters indicate the inserted base
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