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Fig. 3 ZFU6-LentiCRISPRV2-ctgfa mediated gene editing in zebrafish PAC2 cell. A Genomic DNA structure and sgRNA site of ctgfa. B PCR products of ctgfa. M: DL2000 DNA marker; 1: cells transfected with ZFU6-LentiCRISPRV2; 2: cells transfected with ZFU6-LentiCRISPRV2-ctgfa. C Sequencing of ctgfa gene from the puromycin-selection cells transfected with ZFU6-LentiCRISPRV2-ctgfa. D The mutation type of ctgfa gene. Dash represents the deleted base, red letters indicate the CRISPR target site, green letters indicate the PAM sites and blue letters indicate the inserted base
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