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Fig. 5 Loss of SCV phenotype in N315 L3 isolates associated with SigB inactivation. (A) Inactivation of sigB was detected in isolates from passage cycles 4, 20, and 32, and in single L3 white isolates. (B) Identification of sigB truncations and deletions among publicly available S. aureus sequences. Representation of deletions (dark blue), insertions (light blue), and early-stop codons (red) in the 134 S. aureus genomes from the Staphopia database with changes in sigB length. The mutation is numbered when identified more than once. (C) Identification of mutations in the sigB operon among clinical S. aureus isolates exclusively associated with SigB-inactivation. Frequency of specific mutations in sigB operon genes among S. aureus clinical isolates with a SigB-inactivation (as in 5B) compared with 118 controls.