ZFIN Image: Wang et al., 2024, Fig. 5

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Figure Caption

Fig. 5

Muscone suppresses VEGFR2/PI3K/Akt/MAPK axis molecule activation in HUVECs. A Western blot assessment of VEGFR2, PLCγ1, Akt, JNK and ERK protein phosphorylation in HUVECs treated with TCM from muscone-pretreated MDA-MB-231 (231TCM) or BT-549 cells (549TCM). B–D Pharmacological inhibition of VEGFR signaling in HUVECs as determined by Western blot assessment. Cells were pre-exposed to the VEGFR2 inhibitor SU5416 (B), PI3K inhibitor LY294002 (C) or MAPK inhibitor PD98509 (D) for 1 h prior to stimulation with VEGF and muscone. E–G The effects of co-treatment with muscone and SU5416, LY294002, or PD98059 on VEGF-stimulated tube formation of HUVECs. The total tubular structure length was imaged and quantified via Fiji imaging software; Scale bar, 100 μm. Data provided as mean ± SEM. n = 3. *P < 0.05, **P < 0.01, and ***P < 0.001

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