Fig. 1

Fig. 1 Skeletal representation of the intrahepatic biliary network at 5 dpf in mutants recovered from the forward genetic screen. Skeletal representation of the intrahepatic biliary network in mutant larvae based on Tg(Tp1-MmHbb:EGFP)um14 expression at 5 dpf, unbiasedly classified based on branching sub-parameters of the intrahepatic biliary network. (A) Wild-type control (Black border). (B–G) Class Ia (Dark Blue border): lri24 (GK-1.4) (B), lri14 (BJ-1.2) (C), lri25 (HK-1.7) (D), lri36 (JX-2.14) (E), lri11 (AK-13.4) (F), and lri16 (BJ-3.7) (G) mutants belong to this class. (H–Q) Class Ib (Light Blue border): lri31 (IZ-1.6) (H), lri21 (FG-9.3) (I), lri30 (IL-4.1) (J), lri29 (IL-2.3) (K), lri17 (CD-1.4) (L), lri18 (DK-3.2) (M), lri26 (HZ-1.4) (N), lri35 (JW-1.10) (O), lri12 (AK-8.5) (P), and lri37 (KL-10.6) (Q) mutants belong to this class. (R–V) Class II (Red border): lri33 (JL-7.2) (R), lri15 (BJ-2.5) (S), lri34 (JL-9.3) (T), lri13 (BG-1.2) (U), and lri19 (DK-3.4) (V) mutants belong to this class. (W–Y) Class III (Green border): lri20 (EJ-3.10) (W), lri27 (IK-1.6) (X), and lri38 (KZ-3.1) (Y) mutants belong to this class. Ventral views, anterior to the top. The network features are color-coded: end points (green), nodes (white), node-node connections (red), and node-end point connections (yellow). The representative biliary skeleton for each genotype was selected based on the lowest deviation from the average network structural sub-parameter attributes.