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Fig. 4 (+)-1a and (?)-1b inhibited adipogenic differentiation and lipid accumulation in 3T3-L1 adipocytes. (A) Experimental outline of mouse 3T3-L1 fibroblasts differenced with IDIR induction medium. (B) The cell viability was determined by CCK-8 assay. (C) Adipocytes were stained with oil red o and quantified absorbance at 490 nm. (D) Adipocytes were stained with oil red o and visualized under microscopy, scale bar, 200, 50, 25 ?m. (E) Adipocyte differentiation and lipid accumulation were accessed by TG content measurement and (F) GPDH content was determined by manual methods. The data are expressed as mean ± SD (n = 3). ###P < 0.001 vs vehicle, nsP > 0.05, ?P < 0.05, ??P < 0.01, ???P < 0.001 vs IDIR.