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Fig. 4 The MAPK signaling pathway was dysregulated in the combined LAP and DOX exposure-induced cardiotoxicity model. (A-D) Western blotting (A) and quantification of MAPK downstream target proteins, including phosphorylated Jnk (p-Jnk) normalized to total Jnk (B), phosphorylated p38 (p-p38) normalized to total p38 (C) and phosphorylated Erk (p-Erk) normalized to total Erk (D), in the combined LAP and DOX exposure-induced cardiotoxicity model compared to embryos exposed to LAP or DOX alone or DMSO-treated control embryos. N=3–4 biological replicates, one-way ANOVA; *P<0.05, **P<0.01, ***P<0.001.
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