Fig. 5 Antiproliferative effect of mAb and mAb-AMFA. Antiproliferative effect of CTX and CTX-AMFA in A431 (A) and HT-29 (B) cells. A431 and HT-29 cells were treated with 0.5?10 nM CTX or CTX-AMFA for 48 h and cell proliferation was analyzed by MTT assay. Results are presented as the percentage ± SD of living cells compared to control (n=2). (C) Antiproliferative effect of CTX and CTX-AMFA in the presence of EGF. HeLa cells were treated with 0.5?10 nM CTX or CTX-AMFA in the presence of 200 ng/mL EGF for 48 h (n=3). (D) Antiproliferative effect of TRZ or TRZ-AMFA in the presence of EGF. ZR-75?1 cells were treated with 1?50 nM TRZ or TRZ-AMFA in the presence of 200 ng/mL EGF for 72 h (n=2). Cell proliferation was analyzed by MTT assay. Data are expressed as the percentage ± SD of living cells relatively to control cells treated with EGF. Student?s t-test: * p value < 0.05; ** p value < 0.01.
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