ZFIN Image: Xia et al., 2024, Figure 5

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Figure 5

Rapamycin treatment ameliorates the lysosomal defects induced by PIKfyve deficiency in vivo and in vitro. (A–D) Western blot analysis showing the rapamycin treatment restored the elevated LAMP1 level induced by PIKfyve deficiency in zebrafish (A,B) and RAW264.7 cells (C,D). (B) and (D) are densitometric analysis of phosphorylation status of 4E-BP1 and S6K in (A) and (C), respectively (ns, non-significant; * p < 0.05, ** p < 0.01, *** p < 0.001; one-way ANOVA). (E) Lysotracker and LAMP1 staining in zebrafish and RAW264.7 cells. The Lysotracker/LAMP1-positive vacuoles were significantly enlarged in PIKfyve mutant cells and in Apm-treated cells, which were ameliorated by rapamycin treatment.

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