Figure 2

Figure 2

PIKfyve deficiency leads to the accumulation of enlarged LAMP1-positive compartments in macrophages. (A–D) The giant vacuoles in mutant macrophages were revealed to be fused lysosomes by staining with LysoTracker (A) or anti-LAMP1 antibody (C). (B) and (D) respectively show the quantification of LysoTracker and LAMP1 fluorescence intensity (** p < 0.01, **** p < 0.0001). Scale bar, 20 µm. (E) Representative confocal images and tracks of macrophage movement in the CHT of WT and MU zebrafish larvae at 3 dpf. Tracks are coded for speed. Scale bar, 50 µm. (F) Quantification of migration speed of macrophages in (E) (** p < 0.01).