Figure 7

Figure 7

Functional interaction between zebrafish adamtsl4 and cpamd8 genes. (A–D) To obtain double heterozygotes (adamtsl4/+ and cpamd8/+), zebrafish progenitors with heterozygous mutations in both adamtsl4 and cpamd8 genes were crossed. The progeny were genotyped, and the ocular anterior segment was evaluated in adult specimens (2 months) using brightfield microscopy. Sibling wild type (+/+) and single heterozygous (+/−) animals were used as controls. The photographs are representative of the eyes observed. (E,F) The volumes of the anterior segment and the lens within the anterior chamber were calculated as indicated in Methods section. These calculations assumed that the anterior chamber and lens can be represented as spherical caps. In panel (A), the base (r) and height (h) of the spherical caps for the anterior chamber and lens are depicted in yellow and red, respectively. These values were normalized to those of wild type eyes. The following number of eyes were used: Wild type: n = 16; adamtsl4 +/and cpamd8 +/−: n = 16; adamtsl4 +/− cpamd8 +/−: n = 158. **: p < 0.01. ***: p < 0.001.