Figure 1—figure supplement 2.

Figure 1—figure supplement 2. Treatment with human recombinant TGFB1 activates the TGFb pathway in human melanoma cells.

(A) (Left) RNA-sequencing indicated that following a 2-hr treatment with 10 ng/mL TGFB1, 223 genes were significantly up-regulated (q<0.05), including typical TGFb target genes such as SMAD7, JUNB, and PMEPA1, while 94 genes were down-regulated. This Volcano plot indicates top up- and down-regulated genes upon TGFB1 treatment. (Right) Hallmark gene set enrichment analysis (GSEA) of genes ranked by log2 fold-change (log2fc) confirmed that TGFb was the top up-regulated pathway (q=0). RNA-seq was performed in triplicate. (B) TIE:EGFP⁺ melanoma #2 expressing MCR:MCS. TIE:EGFP expression across melanomagenesis. Representative images shown. Illustrated fish diagrams in (B) created with BioRender.com, and published using a CC BY-NC-ND license with permission.