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Fig. 4 Inflammatory cell and gene expression levels in zebrafish infected with Klebsiella pneumoniae and treated under light/dark (L/D) conditions. (A) Neutrophil recruitment of Tg(mpx:EGFP) transgenic zebrafish in the control and NTUH-2044 treatment groups under L/D conditions. (B) Number of neutrophils in Tg(mpx:EGFP) transgenic zebrafish compared with those in the control and NTUH-2044 groups under L/D conditions. (C) Macrophage recruitment of Tg(mpeg:EGFP) transgenic zebrafish in the control and NTUH-2044 groups under L/D conditions. (D) Number of macrophages in Tg(mpeg:EGFP) transgenic zebrafish compared with those in the control and NTUH-2044 groups under L/D conditions. qRT-PCR analysis of inflammation-related genes il1b (E), nfkb (F), tnfa (G), and tgfb1a (H) in the control and NTUH-2044 groups under L/D conditions. Each experiment was repeated three times. Data are expressed as the mean ± standard deviation (SD). Data were analyzed using Student's t-test. (ANOVA) (*P < 0.05, **P < 0.01, ***P < 0.001).