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Rahmati et al., 2024 - Fine-tuning amyloid precursor protein expression through nonsense mediated mRNA decay

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Figure 5.

Expression of app family genes appb^−/− and wild-type (WT) embryos after inhibition of NMD or translation and when knocking down of NMD core factors. A–D, Relative mRNA expression levels of appb, appa, aplp1, and aplp2 in appb^−/− embryos treated with 0.1% DMSO or 10 μM NMDi14 between 24 and 48 hpf (N = 15) to inhibit nonsense-mediated mRNA decay (A,B) or with or without 15 μg/ml CHX between 24 and 29 hpf (N = 15) to block translation (C,D). E, F, Relative mRNA expression levels of appb, appa, aplp1, and aplp2 in wild-type (WT) embryos treated with 0.1% DMSO or 10 μM NMDi14 between 24 and 48 hpf (N = 15) (E) or with or without 15 μg/ml CHX between 24 and 29 hpf (N = 15) (F). A–F, n = 3 biologically independent samples. Data are shown as mean + SD. Student's two-tailed t test were used to calculate p values. *p < 0.05, **p < 0.01, ***p < 0.005, and ****p < 0.0001. ns, nonsignificant.

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