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Fig. 2 T4888M and C643 ATC cell lines display different growth and proliferation rates. (A) Graph showing mass volume μm3 at 1 dpi compared to 4 dpi in ATC cell lines T4888 and C643 (T4888 n=26, C643 n=27). (B) Graph showing mass volume μm3 at 1 dpi compared to 4 dpi tracking individual larva in ATC cell lines T4888 and C643. (C-D) Representative 3D images and reconstruction of ATC tumor masses from 1 dpi to 4 dpi in ATC cell lines T4888 and C643. Images were acquired using the 20x objective. Scale bar= 50 μm. (E) Representative 3D images of EDU+ cells inside cancer mass of ATC cell lines T4888 and C643. (F) Quantification of the total number of EDU+ cells in T4888 and C643 ATC masses (T4888 n=15, C643 n=16). Images were acquired using the 40x objective. Scale bar= 30 μm. (G) Quantification of the number of EDU+ cells normalized by the area μm2 of the ATC mass. Data is from at least two independent experimental replicates. Bar plots show mean ± SEM, each dot represents one larva, P-values are shown in each graph.