Fig. 6

Fig. 6

Multiple hit compounds inhibit lipid ROS accumulation and ferroptosis. A All hit compounds other than dronedarone and quinine are able to inhibit RSL3-induced ferroptosis in HT1080 cells. Data represent mean ± s.d. from three biological repeats and p-value is determined by ordinary one-way ANOVA with Dunnett’s multiple comparisons test. ns, no significance; **, p < 0.01; ***, p < 0.001. (B) FACS analysis of RSL3-induced lipid ROS in cells pretreated with a hit compound. Data represent mean ± s.d. from three biological repeats and p-value is determined by ordinary one-way ANOVA with Dunnett’s multiple comparisons test. ***, p < 0.001. (C) Hit compounds inhibit neomycin-induced lipid ROS accumulation in hair cells. Assays were performed and analyzed as described in Fig. 2B. Scale bar: 20 μm