Fig. 1

Fig. 1

Otoprotection against various ototoxin-induced lateral line neuromast damage by inhibitors of different cell death pathways. Typical confocal images and quantitative results of otoprotection assay. (A) The ferroptosis inhibitor Liproxstatin-1 effectively blocks neomycin-induced hair cell death. The non-specific antioxidant NAC shows partial protective activity while apoptosis inhibitor (Z-VAD-FMK) and necroptosis inhibitor (Necrostatin-1) are not effective in this assay. (B) Liproxstatin-1 and NAC also partially protect against streptomycin-induced hair cell death. (C, D) Gentamicin- or amikacin-induced hair cell death can be partially protected by Liproxstatin-1, Z-VAD-FMK, Necrostatin-1, or NAC. (E) Cisplatin-induced hair cell damage is ameliorated by Z-VAD-FMK, Necrostatin-1, NAC but not Liproxstatin-1. (F) Copper-induce hair cell death is protected by Necrostatin-1 or NAC, but not Liproxstatin-1 or Z-VAD-FMK. Three biological repeats were performed for each assay and at least ten larvae were analyzed for each treatment. Data represent mean ± s.d. and p-value is determined by ordinary one-way ANOVA with Dunnett’s multiple comparisons test. ns, no significance; *, p < 0.05; **, p < 0.01; ***, p < 0.001. Scale bar in A-F: 20 μm