Fig. 3.

Fig. 3.

Reduced viability of embryos carrying the nox2 mutant allele. a) Survival curve of concurrent cohorts of WT and nox2−/− zebrafish embryos. b) Survival curve of concurrent cohorts of WT, nox2+/−, and nox2−/− zebrafish embryos. c) Twenty-four (24) h viability of nox2 WT, heterozygous, and homozygous zebrafish embryos. Panels a, b, and c were performed independently on different days and represent cohorts of genotype-identical embryos assayed in parallel. N-values in brackets (a, b) and within columns (c)—note that although N-values are similar, a and b are independent experiments; +/+, WT; +/−, heterozygous; −/−, homozygous. Log-rank (Mantel–Cox) tests (a, b), Fisher’s exact test (c), P < 0.0001.