Figure 1—figure supplement 2.

Figure 1—figure supplement 2. Partially overlapping expression of rln3a and nmbb in the zebrafish NI.

Fluorescent double-label WISH for rln3a and nmbb transcripts. Dorsal views of 6 dpf larvae, anterior to the top. (A) Z-projection and (A’-A’’’) higher magnification image of NI from larva in A. (B-C’’) NI in two additional larvae. (A’-C’’) Optical sections showing neurons expressing (A’, B, C) rln3a, (A’’, B’, C’) nmbb and (A’’’, B’’, C’’) composite images. White arrowheads indicate neurons that co-express both genes. (A) Scale bar, 100 µm. (A’-C’’) Scale bar, 10 µm. PAG: periaqueductal gray, NI: nucleus incertus.