Fig. 5

Fig. 5 Usp7 Regulates Hh Signaling through Usp7-GMPS Complex (A–A′′) A wing disc expressing gmps RNAi by MS1096 was immunostained to show the expression of Ci (white) and dpp-lacZ (green). gmps knockdown resulted in a decrease of dpp-lacZ (arrow). (B–C′) Wing discs overexpressing usp7 alone (B) or usp7 plus gmps RNAi (C–C′) were immunostained by Fg tag (white) and Ci (green) antibodies. gmps knockdown suppressed the elevation of Ci by Usp7 expression (arrows). (D) S2 cells expressing the indicated proteins were harvested for the two-step immunoprecipitation and analyzed by western blot. (E) GMPS did not bind Ci. The indicated proteins were expressed in S2 cells. The arrowhead indicates IgG. (F) Usp7 interacted with GMPS through its M and C regions in S2 cells. Asterisks mark positions of each protein. (G–I) Wing discs expressing usp7 RNAi alone (G), gmps RNAi alone (H), or usp7 RNAi plus gmps RNAi (I) were immunostained to show ptc-lacZ expression (white). ptc-lacZ expression decreased more apparently when both usp7 and gmps were knocked down (arrows). (J–L) Wing discs overexpressing usp7 alone (J), gmps alone (K), or usp7 plus gmps (L) were immunostained for Ci expression (green). Overexpression of usp7 plus gmps resulted in a more dramatic increase of Ci (arrows). (M–O) Wing discs overexpressing usp7 alone (M), gmps alone (N), or usp7 plus gmps (O) were immunostained to show dpp-lacZ expression (green). Overexpression of usp7 plus gmps resulted in a more dramatic increase in dpp-lacZ expression (arrows).