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Fig. 2 Cell-free expression of membrane proteins fused with N-terminal or C-terminal fluorescent proteins. a) Ten membrane proteins synthesized in cell-free systems. b) Design of membrane protein-fluorescent protein fusion proteins. c) Determining the hydrophobic support and structure of membrane proteins by measuring fluorescence values in supernatant and precipitate. d) Expression of fusion proteins in the cell-free system. Data presented as mean ± SD, n = 3.
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