ZFIN Image: Wu et al., 2019, Fig. 3

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Figure Caption

Fig. 3 PS1–BI1 complex exhibits no proteolytic activity toward the APP-C99 substrate. (A) PS1–BI1 complex failed to generate Aβ42 or Aβ40 using the APP-C99 substrate. The spectroscopic reading of Aβ40 by γ-secretase is normalized as 1.0. The PS1–BI1 complex has no detectable activity. (B) Recombinant BI1 protein exhibits no apparent impact on the production of Aβ42 or Aβ40 by γ-secretase in vitro. Compared with γ-secretase, the amount of BI1 varied from equimolar to a 20-fold molar excess. (C) BI1 at a 200-fold molar excess inhibited the production of Aβ42 or Aβ40 by γ-secretase. The effect is specific because the control protein, AQP-1, at a 200-fold molar excess exhibited no impact on the proteolytic activity of γ-secretase.

Acknowledgments

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