Figure 8—figure supplement 3.

Figure 8—figure supplement 3. Comparisons between ablated and unablated larvae with the same genotype.

Unablated control group only includes only Tg(gsc2:QF2)^c721; Tg(QUAS:GFP)^c578 (A, C, D, E) or Tg(rln3a:QF2, he1.1:YFP)^c836; Tg(QUAS:GFP)^c578 (B, F, G, H) siblings of ablated larvae. (A, B) Mean of distance traveled during 5 s pre- and post-shock. (A) Unablated c721: pre=1.30±0.46 cm, post=5.80±0.61 cm. (B) Unablated c836: pre=0.51±0.18 cm, post=5.96±1.07 cm. (C, F) Mean of distance traveled during the first 115 s of the recording. (C) Unablated c721=19.33±4.59 cm. (F) Unablated c836=20.29±4.55 cm. (D, G) Average length of phases of movement during the pre-shock period, defined as continuous phases of movement with no more than one second of prolonged immobility. (D) Unablated c721=8.28 ± 3.11 s. (G) Unablated c836=6.64±1.07 s. (E, H) Mean number of phases of movement during the pre-shock period. (E) Unablated c721=8.17±1.99. (H) Unablated c836=7.4±1.30. All other groups have the same values as in Figure 8. (A, B) Kruskal-Wallis rank sum test: (A) ***p=4.74 x 10^–15, (B) ***p=2.85 x 10^–16. (A, B) Dunn’s post-hoc tests with adjustment for multiple comparisons show no statistically significant differences within pre- and post-shock epochs, p<0.001*** for each pre-shock vs. post-shock comparison. (C) Kruskal-Wallis rank sum test: **p=0.0012. Dunn’s post-hoc tests with adjustment for multiple comparisons show ablated rln3a NI neurons vs. unablated *p=0.012, ablated rln3a vs. gsc2 NI neurons **p=0.0023, or ablated rln3a NI vs. rln3a PAG neurons **p=0.0023. (D) Kruskal-Wallis rank sum test: **p=0.0018. Dunn’s post-hoc tests with adjustment for multiple comparisons show ablated rln3a NI neurons vs. unablated p=0.098, ablated rln3a vs. gsc2 NI neurons p=0.060, or ablated rln3a NI vs. rln3a PAG neurons ***p=0.00076. (F) Kruskal-Wallis rank sum test: **p=0.0012. Dunn’s post-hoc tests with adjustment for multiple comparisons show ablated rln3a NI neurons vs. unablated **p=0.0078, ablated rln3a vs. gsc2 NI neurons **p=0.0022, or ablated rln3a NI vs. rln3a PAG neurons **p=0.0022. (G) Kruskal-Wallis rank sum test: **p=0.0012. Dunn’s post-hoc tests with adjustment for multiple comparisons show ablated rln3a NI neurons vs. unablated p=0.099, ablated rln3a vs. gsc2 NI neurons ablated *p=0.042, or ablated rln3a NI vs. rln3a PAG neurons ***p=0.00049. (E, H) Kruskal-Wallis rank sum test: (E) p=0.87, (H) p=0.90.