Figure 8—figure supplement 2.

Figure 8—figure supplement 2. Loss of rln3a NI neurons increases turning behavior.

Ratio of the total size, in degrees, of all calculated angles during the first 115 s of the recording, divided by total distance traveled in millimeters. (A) Mean ratio for unablated controls (81.94±12.85), or larvae with ablated gsc2 (81.53±15.81), rln3a NI (182.72±15.29), or rln3a PAG (84.26±16.70) neurons. Kruskal-Wallis rank sum test: ***p=0.00017. Dunn’s post-hoc tests with adjustment for multiple comparisons show ablated rln3a NI neurons vs. unablated ***p=0.00038, ablated rln3a vs. gsc2 NI neurons ***p=0.00066, or ablated rln3a NI vs. rln3a PAG neurons ***p=0.00066. (B, C) Unablated control group includes only (B) Tg(gsc2:QF2)^c721; Tg(QUAS:GFP)^c578 or (C) Tg(rln3a:QF2, he1.1:YFP)^c836; Tg(QUAS:GFP)^c578 siblings of ablated larvae. Mean ratios: (B) Unablated c721=66.93±15.38. (C) Unablated c836=93.95±19.50. All other groups have the same values as in A. (B) Kruskal-Wallis rank sum test: ***p=0.000097. Dunn’s post-hoc tests with adjustment for multiple comparisons show ablated rln3a NI neurons vs. unablated ***p=0.00057, ablated rln3a vs. gsc2 NI neurons ***p=0.00059, or ablated rln3a NI vs. rln3a PAG neurons ***p=0.00061. (C) Kruskal-Wallis rank sum test: ***p=0.00045. Dunn’s post-hoc tests with adjustment for multiple comparisons show ablated rln3a NI neurons vs. unablated **p=0.0045, ablated rln3a vs. gsc2 NI neurons **p=0.001, or ablated rln3a NI vs. rln3a PAG neurons **p=0.001.