Figure 8—figure supplement 1.

Figure 8—figure supplement 1. Confirmation of selective ablation of NI neuronal clusters.

(A-D’) WISH for (A, A’, C, C’) gsc2 or (B, B’, D, D’) rln3a was performed on 7 dpf larvae. (A’, B’) Tg(gsc2:QF2)^c721; Tg(QUAS:GFP)^c578 larvae whose gsc2 neurons were ablated at 6 dpf. (C’, D’) Tg(rln3a:QF2, he1.1:YFP)^c836; Tg(QUAS:GFP)^c578 larvae whose rln3a NI neurons were ablated at 6 dpf. (A, B, C, D) Unablated sibling controls for larvae in A’, B’, C’ and D’ respectively. (D, D’) Higher background due to the longer incubation time required to detect rln3a transcripts, which are reduced in Tg(rln3a:QF2, he1.1:YFP)^c836 heterozygotes relative to wild type. Dorsal views, anterior to the top.