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Figure 3.

ID: ZDB-IMAGE-240603-103
Publication: Spikol et al., 2024 - Genetically defined nucleus incertus neurons differ in connectivity and function
All Figures: Figures for Spikol et al., 2024

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Figure Caption

Figure 3. rln3a and gsc2 NI neurons are largely GABAergic.

(A-F’’) Confocal images of 6 dpf larvae. (A–C) Lateral views, anterior left. (D-F’’) Dorsal views, anterior to the top. (A) Z-projection of Tg(gsc2:QF2)^c721; Tg(QUAS:GFP)^c578; Tg(slc17a6b:DsRed)^nns9 larva. (B-F’’) Optical sections. (B) PAG and (C) NI of a Tg(rln3a:QF2, he1.1:YFP)^c836; Tg(QUAS:mApple, he1.1:CFP)^c788; Tg(slc17a6b:GFP)^zf139 larva. (D) Tg(gsc2:QF2)^c721; Tg(QUAS:mApple-CAAX, he1.1:mCherry)^c636; TgBac(gad1b:GFP)^nns25 larva. (D’) Magnified view of boxed region in D. White arrowhead indicates a gad1b and gsc2 co-expressing neuron. (E-F’’) Tg(rln3a:QF2, he1.1:YFP)^c836; Tg(QUAS:mApple, he1.1:CFP)^c788; TgBac(gad1b:GFP)^nns25 larva. (E-E’’) View of PAG. (F-F’’) View of NI. (E’, F’) Magnified views of boxed regions in E and F. (E’’, F’’) Individual neurons indicated by arrowheads in E’ and F’, respectively. Top panels: GABAergic, middle panels: rln3a, bottom panels: composite. (G) Boxplot showing the percentage of gsc2 and rln3a NI neurons, and rln3a PAG neurons that express TgBac(gad1b:GFP)^nns25, n=3 larvae. Scale bars, 100 µm.

Acknowledgments

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