Figure 2—figure supplement 2.

Figure 2—figure supplement 2. gsc2 neurons reside outside the IPN in the adult brain.

Drawing of adult zebrafish brain in lateral view (after Wullimann et al., 1996), indicating positions of sections shown in (B-D’). (B-D’) Two confocal optical sections are shown for each of three 70 µm vibratome slices from a representative TgBAC(gng8:Eco.NfsB-2A-CAAX-GFP)^c375; Tg(gsc2:QF2)^c721; Tg(QUAS:NLS-mApple)^c718 adult brain. Sections are ordered from rostral to caudal with anterior to the top. Dashed lines delineate ventricles and the medial longitudinal fascicles (MLF). Arrowheads indicate mApple labeling of gsc2 neuronal projections to the hypothalamus in B and B’ and gsc2 cell bodies in C-D. Scale bar, 100 µm. Ob: olfactory bulb, Tel: telencephalon, Hb: habenula, OT: optic tectum, IPN: interpeduncular nucleus, Ce: cerebellum, Md: medulla.