ZFIN Image: Lai et al., 2024, Fig. 5

Image

Figure Caption

Fig. 5 Upregulation of endogenous targets of nonsense-mediated mRNA decay (NMD) in Smg9-deficient zebrafish.

a–j Quantitative PCR analysis of endogenous target genes of NMD in smg9^+/+ and smg9^oi7/oi7 larvae at 14 dpf. k Western blot analysis of lysates from smg9^+/+ and smg9^oi7/oi7 larvae at 2 dpf to determine the total FLAG-UPF1 (UPF1) and phosphorylated FLAG-UPF1(p-UPF1) levels. l Quantification of the total FLAG-UPF1 (UPF1) and phosphorylated FLAG-UPF1 (p-UPF1) levels in three independent experiments. Error bars indicate SD. *P < 0.05, ** P < 0.01, and *** P < 0.001. ns: not significant.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Commun Biol