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Fig. 5

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ZDB-IMAGE-240531-140
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Lai et al., 2024 - Effect of nonsense-mediated mRNA decay factor SMG9 deficiency on premature aging in zebrafish
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Fig. 5 Upregulation of endogenous targets of nonsense-mediated mRNA decay (NMD) in Smg9-deficient zebrafish.

a–j Quantitative PCR analysis of endogenous target genes of NMD in smg9+/+ and smg9oi7/oi7 larvae at 14 dpf. k Western blot analysis of lysates from smg9+/+ and smg9oi7/oi7 larvae at 2 dpf to determine the total FLAG-UPF1 (UPF1) and phosphorylated FLAG-UPF1(p-UPF1) levels. l Quantification of the total FLAG-UPF1 (UPF1) and phosphorylated FLAG-UPF1 (p-UPF1) levels in three independent experiments. Error bars indicate SD. *P < 0.05, ** P < 0.01, and *** P < 0.001. ns: not significant.

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