Fig. 2

Fig. 2 Impaired cardiac contractions in Smg9-deficient zebrafish.

a Representative image of the heart of cmlc2:EGFP zebrafish. The red and white dotted lines indicate the atrium and ventricle, respectively. b Quantification of the atrium and ventricle areas in smg9^+/+ and smg9^oi7/oi7 zebrafish at 6 dpf. c Representative images of hematoxylin and eosin staining of smg9^+/+ and smg9^oi7/oi7 hearts at 6 dpf. d Representative dynamic pixel change patterns of the heart in cmlc2:EGFP zebrafish, showing the heartbeat and cardiac contraction of smg9^+/+ and smg9^oi7/oi7 zebrafish. e Quantification of cardiac contractions in smg9^+/+ and smg9^oi7/oi7 zebrafish larvae at 6 dpf. f Quantification of the heart rates of smg9^+/+ and smg9^oi7/oi7 zebrafish larvae at 6 dpf. g Quantification of cardiac contractions of 6 dpf smg9^+/+ and smg9^oi7/oi7 larvae injected with control (con-in) or zebrafish smg9 mRNA (smg9-in). Error bars indicate SD. bpm: beats per minute. *P < 0.05, **P < 0.01, and ***P < 0.001. ns: not significant.