|
Fig. 6 The integrated analysis of transcriptomic and metabolomic profiles following wedelolactone (WEL) and demethylwedelolactone (DWEL) treatment. (A) Single-sample gene set enrichment analysis (ssGSEA) of the key metabolic pathway across each sample. (B) Gene set enrichment analysis (GSEA) of steroid biosynthesis and fatty acid elongation in WEL and DWEL treatment group vs. non-alcoholic fatty liver disease (NAFLD) group. (C) The biological processes of steroid synthesis, fatty acid metabolism, and glutathione metabolism, and the crucial genes involved. (D) The relative mRNA levels of genes in essential metabolic pathways determined by quantitative real-time PCR (qRT-PCR). Data are presented as mean ± standard deviation (SD) (n = 10). ?P < 0.05, ???P < 0.001, ns: no significance. NES: normalized enrichment score; FDR: false discovery rate; TAA: thioacetamide; farnesyl-PP: farnesyl pyrophosphate.