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Fig. 3 The metabolism and spatial signatures of wedelolactone (WEL) and demethylwedelolactone (DWEL) in zebrafish. (A) Metabolism pathway of WEL and the structural inferences of its metabolites. (B) Mass spectrometry (MS) images depicting the spatial distribution of WEL and its metabolites in zebrafish whole-body section. (C) Metabolism pathway of DWEL and the structural inferences of its metabolites. (D) MS images depicting the spatial distribution of DWEL and its metabolites in zebrafish. WEL-Sulf: sulfated wedelolactone; WEL-GlcA: glucuronidated wedelolactone; WEL-Sulf-GlcA: sulfated-glucuronidated wedelolactone; WEL-Sulf-Glc: sulfated-glucosidated wedelolactone; DWEL-Sulf: sulfated demethylwedelolactone; DWEL-GlcA: glucuronidated demethylwedelolactone; DWEL-Sulf-GlcA: sulfated-glucuronidated demethylwedelolactone; DWEL-Sulf-Glc: sulfated-glucosidated demethylwedelolactone; MWEL: methylated wedelolactone; MWEL-Sulf: methylated and sulfated wedelolactone; MWEL-GlcA: methylated and glucuronidated wedelolactone; MWEL-Sulf-GlcA: methylated, sulfated and glucuronidated wedelolactone; MWEL-Sulf-Glc: methylated, sulfated and glucosidated wedelolactone.