Figure 3

Figure 3

Increased Ca²⁺ levels, Ca²⁺ transient amplitude, and bradycardia in 3 dpf Tg(myl7:mCyRFP1-GCaMP6f) larvae injected with tnnt2a MO. (A) Ratiometric images of hearts and atrial and ventricular Ca²⁺ traces from representative 3 dpf Tg(myl7: mCyRFP1-GCaMP6f) sibling and tnnt2a morphant larvae. The image of siblings shows the mechanical systole of the ventricle. (B) Atrial and ventricular systolic Ca²⁺ (mCyRFP1-GCaMP6f emission ratio), diastolic Ca²⁺, and Ca²⁺ transient amplitude in sibling (n = 23) and tnnt2a morphant (n = 28) larvae. (C) Atrial CaT frequency (min⁻¹) in these larvae. Statistical analysis was performed using an unpaired Student’s t-test and the Mann–Whitney test for non-parametric data. Data are shown as the mean ± SD (** for p < 0.01 and **** p < 0.0001 for parametric statistics; ^xx for p < 0.01, ^xxxp < 0.001, and ^xxxx p < 0.0001 for non-parametric statistics).