ZFIN Image: Peng et al., 2024, Figure 4

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Figure 4

The influence of esr2b knockout on sex ratio and the number of PGCs during development. (A). Sex ratios of esr2b knockout zebrafish and wild-type zebrafish at 90 dpf. Orange and blue represents the proportion of male and female zebrafish in the group, respectively. (B) Whole-mount in situ hybridization with vasa mRNA probe during early development. At 4.3 hpf, also named the dome stage, the average number of vasa signals in wild-type and esr2b KO embryos were 10 (n = 15) and 9.8 (n = 15) (B1,B2); At 10 hpf, also named the bud stage, the average number of vasa signals in wild-type and esr2b KO embryos were 22.9 (n = 15) and 25 (n = 15) (B3,B4); At 24 hpf, the vasa signal intensity showed no significant difference (B5,B6). (C) Comparison of the number of PGCs between wild-type and esr2b knockout zebrafish at 4.3 hpf and 10 hpf. n.s.; not significant. **, p < 0.01.

Acknowledgments

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