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Fig. 2 Phenotypic Effects of Different Isomeric Compositions of Plinabulin on Zebrafish Embryos for Sublethal Concentrations. A) negative control (DMSO 0.125 %) – No abnormal phenotype observed. B) 0.1 μM Z-1 (LD50=0.31 μM) – Severe malformation evident in embryos. C) 10 μM E-1 (LD50>7.5 μM) – Embryos exhibit normal appearance with no malformations or deaths. D) 0.3 μM E-1 irradiated with cyan light (490 nm) (LD50>1 μM for that mixture) – Embryos display malformation. Three representative embryos are depicted for each scenario. All pictures taken at 24 hpf. Scale bar: 500 μm.
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