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Fig. 3 Expression of Cre and EGFP in zTg(cryaa:Cre-cryaa:EGFP) zebrafish. A: Whole-mount in situ hybridization (WISH) of cryaa, egfp, and cre mRNA in 48 and 72 hpf WT and zTg(cryaa:Cre-cryaa:EGFP; Tg) zebrafish. B: Cryosection of 72 hpf Tg embryos of A. C: Fluorescent EGFP was photographed in the lenses of zTg(cryaa:Cre-cryaa:EGFP) zebrafish at the 20 dpf, 1 mpf, 3 mpf, 6 mpf, 9 mpf, and 12 mpf stages. (D) Immunoblot showing the expression of Cre protein and Gapdh in the lens, lens-excluded eye tissue, and eyes-excluded body tissues of WT and zTg(cryaa:Cre-cryaa:EGFP) zebrafish at 15 dpf.
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