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Fig. 5 Effects of Sirt1-blockade on Mapk-activation. (A) Representative immunoblotting data. The activation level of Mapk was monitored by measuring relative levels of phospho-Erk1/2. Stage-matched Norm and Reoxy embryos were similarly treated with 50 µM EX-527 and subjected to immunoblot analysis. (B) Quantification data of relative Erk1/2 phosphorylation level. The value of control group (DMSO) was set as 1.0. Values shown are mean ± SD. Data were obtained from three–five independent assays. **: P < 0.01, ns: P > 0.05.
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