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Fig. 3 Effect of specific blockade of sirt2 on embryonic growth. (A) Changes in growth rate of sirt2-MO-injected embryos. The relative value is shown. The control group was set as 100. Values are mean ± SD. Data were obtained from two–three independent assays. n = 21 (Norm ctr-MO); n = 10 (Norm sirt2-MO); n = 39 (Reoxy ctr-MO); n = 26 (Reoxy sirt2-MO). **: P < 0.01, ns: P > 0.05. (B) Changes in growth rate of the AK-1 (Sirt2-specific inhibitor)-treated embryos in Norm and Reoxy conditions. The control group (DMSO) was set as 100. Values shown are mean ± SD. Data were obtained from two independent assays. n = 36 (Norm DMSO); n = 36 (Norm AK-1 10 µM); n = 34 (Norm AK-1 50 µM); n = 34 (Norm AK-1 100 µM); n = 32 (Reoxy DMSO); n = 30 (Reoxy AK-1 10 µM); n = 30 (Reoxy AK-1 50 µM); n = 31 (Reoxy AK-1 100 µM). The same letter means statistically not significantly different (P > 0.05).