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Fig. 2 Effect of specific blockade of sirt1 on embryonic growth. (A) Changes in growth rate of sirt1-MO-injected embryos. The relative value is shown. The control group was set as 100. Values are mean ± SD. Data were obtained from three independent assays. n = 30 (Norm ctr-MO); n = 31 (Norm sirt1-MO); n = 37 (Reoxy ctr-MO); n = 32 (Reoxy sirt1-MO). **: P < 0.01; ns: P > 0.05. (B) Changes in growth rate of the EX-527 (Sirt1-specific inhibitor)-treated embryos in Norm and Reoxy conditions. The control group (DMSO) was set as 100. Values shown are mean ± SD. Data were obtained from two–seven independent assays. n = 48 (Norm DMSO); n = 28 (Norm EX-527 10 µM); n = 48 (Norm EX-527 50 µM); n = 76 (Reoxy DMSO); n = 72 (DMSO EX-527 10 µM); n = 59 (DMSO EX-527 50 µM). Different letters mean statistical significance at P < 0.05.