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Fig. 1 Temporal blockade of Sirt1/2 in embryos growing under constant normal oxygen condition (Norm) and re-oxygenation condition (Reoxy). (A) A diagram showing outline of drug exposure experiments. Sirtinol simultaneously inhibits both Sirt1 and Sirt2 in Norm and Reoxy conditions. Embryos in comparable growth stages were treated with Sirtinol, and the growth rate was analyzed. (B) Changes in growth rate of the Sirtinol-treated embryos in Norm and Reoxy conditions. The control group (DMSO) was set as 100. Values shown are mean ± SD. Data were obtained from two–four independent assays. n = 47 (Norm DMSO); n = 27 (Norm Sirtinol 10 µM); n = 22 (Norm Sirtinol 25 µM); n = 35 (Norm Sirtinol 50 µM); n = 74 (Reoxy DMSO); n = 31 (Reoxy Sirtinol 10 µM); n = 20 (Reoxy Sirtinol 25 µM); n = 57 (Reoxy Sirtinol 50 µM). Different letters mean statistical significance at P < 0.0001.